The invention provides a regenerating method for a polyvinylidene fluoride separation membrane carrying a thiourea group and used for absorbing silver ions. The regenerating method comprises the following steps: firstly, performing alkalization treatment on a polyvinylidene fluoride microsphere, thereby generating a carbon-carbon double bond in a molecular structure thereof; introducing 4-chloromethyl styrene into a polyvinylidene fluoride molecular chain through a chemical grafting process; utilizing a chemical bonding action between the introduced thiourea group and the thiourea molecule tograft the thiourea group onto a polyvinylidene fluoride skeleton; adopting a phase transfer process for preparing the polyvinylidene fluoride separation membrane carrying the thiourea group. The regenerating method is simple in preparation process and is easy for industrial production; the thiourea group introduced into the separation membrane is stable and is difficult to leach and lose; the prepared separation membrane has excellent absorbing selectivity to the silver ions in effluent; the regenerating eluant of separation membrane is prepared for separating the silver ions from the separation membrane, so as to realize regeneration and recycling of the separation membrane; the invention also provides the method for recycling the silver ions and regenerating the regenerating eluant of separation membrane.