The invention discloses a hard disk encryption key storage method, device and equipment and a readable storage medium, and the method comprises the steps: determining a hard disk corresponding to a hard disk encryption request as a target hard disk after a terminal detects the hard disk encryption request; calling a preset encryption program to generate an initial key for encrypting the target hard disk; and calling a BIOS interface program of a basic input/output system to store the initial key into a reserved storage space of the BIOS chip. On the premise that hardware cost is not added, thestorage space for storing the secret key is provided, and the terminal can automatically decrypt the startup of the hard disk under the condition that the terminal does not have the TPM.