The invention provides a method for tailing pond microbial remediation with multiple sulfate-reducing bacteria EM2, SRB-4 and iron ion reducing bacteria Biometek-A. The above bacteria are introduced into tailings and nutrients needed by microbial growth are added to construct a set of tailing pond microbial remediation system. Some nutrients added into the tailings and growth of the reducing bacteria can inhibit the growth of inherent oxidizing bacteria in tailings and obviously inhibit the oxidation and dissolution of multiple heavy metal ions, thus improving the pH of tailings, lowering theredox potential, and at the same time greatly reducing the permeability of tailings. Therefore, the remediation method can reach a remediation effect on the tailing pond.