The invention discloses a precious metal ore flotation reagent and application thereof. The precious metal ore flotation reagent comprise, by weight, the following components: 1-25 parts of a collector A, 1-15 parts of a collector B and 1-8 parts of a foaming agent, wherein the collector A is selected from at least one of xanthate collectors, aerofloat collectors and ester collectors; the collector B is selected from at least one of triphenylphosphine and triphenyl phosphine oxide. Compared with traditional xanthate collectors, aerofloat collectors, ester collectors and combined collectors thereof, the flotation reagent can greatly improve the flotation recovery rate of precious metal ores on the premise of ensuring the concentrate grade, and meanwhile the dosage of the reagent is obviously lower than those of the traditional reagents and has stronger collecting capacity on precious metals and higher efficiency, and the dosage of the reagent is saved and the cost of the flotation reagent is reduced while the flotation recovery rate of precious metal ores is improved.