The invention belongs to the field of feed additives and relates to an obsolete broiler meat quality improving preparation and a preparation method and application thereof. The meat quality improvingpreparation comprises rhodotorula mucilaginosa, saccharomyces cerevisiae, bacillus subtilis, vitamin E and plant essential oil. The preparation method includes: well mixing components. The improving preparation is added into livestock-poultry feed according to an adding amount of 0.1-0.3% of total weight of the feed. The improving preparation can serve as an antibiotic substitute to eliminate drugresidue, improve broiler meat and egg product quality and remarkably increase content of inosinic acid and intermuscular fat in broiler muscles to enable broiler meat to be smooth, tender and tasty;the improving preparation can also be used for preventing and controlling broiler diarrhea, improving immunity and disease resistance and lowering death and culling rate of broilers in the egg layingperiod, can improve feed conversion ate and egg laying performance and is conducive to purifying henhouse environment and protecting and improving ecological environment.