The invention provides a quick cleaning method for entomopathogenic nematodes. The method comprises the following steps: placing a culture mixture obtained by an entomopathogenic nematode solid culture method into a washing bucket of a washing machine, setting washing program of the washing machine, sequentially performing single-time washing, dehydrating, rinsing, dehydrating or single-time washing, dehydrating, rinsing, dehydrating and dehydrating, and collecting washing machine discharge liquor; leaving the obtained discharge liquor to stand for precipitation, and discarding supernatant; stirring and cleaning precipitates with distilled water to obtain entomopathogenic nematode concentrated liquor; and finally, adding benzisothiazolinone-3-ketone, using sponge to adsorb, and storing ata low temperature after sealing and packaging. The method is simple, adopts easily available instruments, can effectively improve recycling efficiency of nematodes, shortens recycling time, is small in damage on nematodes, improves quality of nematodes, is high in survival rate of the obtained nematodes, and is high in invasiveness.