The invention relates to a railway noise barrier fatigue testing machine, and belongs to the technical field of vibration reduction and noise reduction. The railway noise barrier fatigue testing machine comprises a servo hydraulic source, a servo actuator, a noise barrier test piece clamp, a sensor and a control system; the servo hydraulic source is connected with the servo actuator through an oilinlet pipe and an oil outlet pipe; the sensor is positioned on the servo actuator; the servo actuator acts on a noise barrier test piece through the noise barrier test piece clamp; and the control system is connected with the servo hydraulic source, the servo actuator and the sensor through a control cable. The fatigue testing machine can simulate a working condition that a pulsation wind pressure of a train generates a tension and compression circulation effect on the noise barrier test piece; the requirements on the noise barrier test pieces with different lengths and widths can be met; anda combined device of a pressure plate and four or more micro-arc surface force applying plates of the test piece clamp is used for transferring a load, thereby reducing pressure concentration phenomena generated when a single plane type force applying plate applies force to the noise barrier test piece.