The invention discloses a preparation method of a high-property anion exchange medium. The preparation method comprises the following steps: S1, agarose microspheres are swelled in alkali liquor; S2,allyl glycidyl ether is added into the alkali liquor to activate the agarose microspheres, and then the activated agarose microspheres are bromized; S3, the bromized agarose microspheres carry out a grafting reaction with glucan under the alkaline condition, and agarose microspheres grafted with the glucan are obtained; and S4, the agarose microspheres grafted with the glucan are coupled with anion exchange ligands, and the high-property anion exchange medium is obtained. The invention further discloses the high-property anion exchange medium prepared through the method. The high-property anion exchange medium has the advantages of being high in protein loading capacity, high in operating flow velocity, good in mechanical strength and the like.