The invention discloses a drosophila melanogaster luring and killing agent and a preparation method. The luring and killing agent is obtained by adopting and uniformly mixing raw materials of 5%-7% ofbrown sugar, 5%-7% of edible vinegar, 1%-2% of pineapple essence and the balance water. The luring and killing agent has the advantages that the components of the luring and killing agent are simple,the obtaining of the components is easy, the cost is low, the environment is not polluted, the quality of red raspberry fruits is not influenced, and the quality ensuring, quantity ensuring, yield improving and yield stabilizing of an orchard can be achieved.