The invention discloses a reagent bottle cleaning device. The reagent bottle cleaning device comprises a basal body; a cleaning module is arranged at the top of the basal body; the cleaning module comprises multiple pushing cylinders with downward piston rods; the piston rods of the pushing cylinders are provided with mounting plates matched with the basal body; multiple mounting grooves are formed in the mounting plates; cleaning motors with downward output shafts are arranged in the mounting grooves; cleaning screw rods are arranged on output shafts of the cleaning motors; a cleaning block matched with the cleaning module is arranged at the bottom of the basal body; multiple cleaning grooves corresponding to the cleaning screw rods are formed in the cleaning block; multiple injecting pumps are arranged between the cleaning block and the cleaning module; the injecting pumps are connected with the basal body through lifting cylinders; and under the effect of a main control chip, the full-automatic quick cleaning of reagent bottles can be realized, the working period is shortened, and the cleaning efficiency is improved.