The invention discloses an audio and video synchronization method and device and a storage medium, which are applied to an audio track or a video track. The audio and video synchronization method comprises the following steps: when the identification information of a current sample wafer of a current track is equal to the identification information of a reference sample wafer, judging whether thecheck value of the current sample wafer is equal to the check value of the reference sample wafer or not; When the check value of the current sample wafer is not equal to the check value of the reference sample wafer, obtaining a target sample wafer equal to the check value of the reference sample wafer; And assigning the identification information of the reference sample to the target sample. According to the technical scheme, the problem of audio and video asynchronization can be solved.