The invention provides a preparation method of a hollow carbon nanosphere. The hollow carbon nanosphere is prepared from acorn kernel powder serving as a carbon source and nickel acetate serving as anickel source with a calcined carbon method. The method is simple, the process is easy to control, the acorn kernel powder is wide in source and low in cost, and the prepared hollow carbon nanosphereis regular in structure, uniform in particle size which is 30 to 50 nm, high in absorbility and large in specific surface area. The method is suitable for preparing the hollow carbon nanosphere.