The invention discloses a pluggable roll printing coil probe of a nuclear magnetic resonance instrument and a design method thereof, which relates to the nuclear magnetic resonance instrument. The probe is provided with a probe base, a tuning matching circuit, a fixing hole provided with a shielding layer shimming coil, a sample tube, a support tube, a roll printing coil, a coil upper silver ring,a coil lower silver ring, an upper elastic metal contact, a lower elastic metal contact, a force column, a tuning capacitor, a matching capacitor and conductor. The conductive silver is offset printed on the surface of the sample tube by adopting a rolling printing method, so that the manufacturing of the rolling printing coil can be completed quickly, the processing technology of printing and electroplating is avoided, and the manufacturing technology of the rolling printing coil is simplified. According to the design requirement, a printing model of different types of roll printing coils isdesigned on a photoetching substrate, conductive silver glue is attached to different types of sample tubes by using a roll printing method, and the roll printing coils with various geometric shapesand types, such as saddle-shaped coils, AG coils and birdcage coils and the like, are manufactured to extend the flexibility and applicability of the roll printing coil design.