The invention discloses an isolation diagnosis platform based on air curtain isolation. The isolation diagnosis platform comprises a control mainboard, a top cover, two fan boxes, a working table anda supporting frame, wherein a circulating air duct is formed among the top cover, the two fan boxes and the supporting frame, the working table is arranged between the two fan boxes and the supportingframe, a strip-shaped air outlet is formed in the working table, thus an air curtain is formed after air of the circulating air duct passing through the strip-shaped air outlet, and the function of directly converting exhaled gas of patients into a partition air curtain is achieved. And concentration information of PM2.5 in the current air is collected by a sensor, the collected concentration information of PM2.5 is sent to the control mainboard for sending a control instruction to a fan control box, so as to adjust the air outlet speed of the air curtain by adjusting the rotating speed of afan. By adopting the embodiment of the isolation diagnosis platform based on air curtain isolation, the air curtain can be automatically controlled according to the current air quality, when the number of people increases, the isolation effect is improved, when the number of people decreases, the air outlet volume of the fan can be adjusted, and thus energy conservation and environmental protection are achieved.