The invention discloses sun-proof bacterium-inhibition thermo-sensitive paper. According to the technical scheme, the paper is characterized by comprising a raw paper layer, a thermosensitive color-change layer and a protection layer, the thermosensitive color-change layer is clamped between the raw paper layer and the protection layer, and sun-proof bacterium-inhibition layers are arranged on thefront and reverse faces of the raw paper layer; each sun-proof bacterium-inhibition layer is prepared from, by weight, 80-100 parts of an acrylate adhesive, 30-40 parts of a nano inorganic filler, 50-60 parts of an organic solvent, 5-8 parts of a sun-screening agent, 1-5 parts of an aloe extraction solution, 1-5 parts of an antioxidant and 1-3 parts of beewax; the organic solvent capable of dissolving beewax is selected as the solvent. The thermo-sensitive paper has the advantages of being excellent in sun-proof performance, capable of achieving a durable color after color development, good in bacterium-inhibition performance and wide in application range. The invention further correspondingly discloses a preparation method of the thermo-sensitive paper.