The invention discloses a three-dimensional cross biomimetic model for magnetic resonance diffusion tensor imaging reconstruction algorithm verification and a preparation method thereof. A coaxial electrospinning technology is combined with a six-degree-of-freedom mechanical arm, multiple coplanar cross and different plane cross fiber bundles formed by stacking electrospun hollow fibers, each fiber bundle is formed by N electrospun hollow fibers, the outer diameter of each electrospun hollow fiber is 1-20 micrometer, an outer shell of each electrospun hollow fiber is made of a water-insolublepolymer material, and a core material is a water-soluble polymer material; then the obtained fiber bundles are soaked in pure water, the core material is removed, and the final three-dimensional crossbiomimetic model can be obtained. The model can serve as an objective model which is of the known structure and is validated by a magnetic resonance diffusion tensor imaging reconstruction algorithm.