The invention discloses a Seneca valley virus structural protein epitope polypeptide and application thereof. The polypeptide is the polypeptide represented by a sequence 1 in a sequence table, a sequence 2 in the sequence table, a sequence 3 in the sequence table or a sequence 4 in the sequence table. The Seneca valley virus structural protein epitope polypeptide is used for preparing a chemically synthesized antigen peptide coated elisa plate used for a kit, is low in antigen dosage and high in sensitivity and specificity, can effectively detect whether a structural protein antibody infectedwith a Seneca valley virus exists or not, is high in sensitivity, good in specificity and fast and convenient to operate and has a good market prospect.