The invention provides a preparation process of drug containing a diammonium glycyrrhizinate compound and belongs to the technical field of medicines and preparation thereof. The preparation process contains a concentrated Chinese herbal medicine water decoction extract and diammonium glycyrrhizinate and comprises the steps as follows: (1) preparing the concentrated Chinese herbal medicine water decoction extract, wherein the step includes the sub-steps of A taking and soaking Chinese herbal medicines in distilled water, heating, decocting, filtering and storing the filtrate, B adding distilled water into filtered dregs for decoction and filtration and storing the filtrate, C mixing the two filtrates for concentration; (2) preparing a diuretic acid solution; (3) mixing the concentrated Chinese herbal medicine water decoction extract, the diammonium glycyrrhizinate and the diuretic acid solution. The drug prepared by the preparation process and containing the diammonium glycyrrhizinatecompound improves the symptoms for chronic hepatitis B by utilizing the synergistic effect of Chinese herbal medicine and western medicine.