The invention belongs to the field of planting of edible fungi and particularly relates to a culture box for fungi. The culture box for fungi comprises an upper box wall, a lower box wall, a left boxwall, a right box wall, a front box wall and a rear box wall. A horizontal partition plate is connected between the left box wall and the right box wall and located on the horizontal symmetry axis ofthe culture box for fungi and divides the culture box for fungi into an upper constant-temperature chamber and a lower planting chamber, a horizontally-placed constant-temperature modulator tube is installed in the upper constant-temperature chamber, an opening is formed in the middle of the horizontal partition plate, and a temperature adjustment device is clamped to the opening. According to thetechnical scheme, the temperature difference in the culture box is sensed through the temperature adjustment device, the temperature is adjusted, and the culture box for fungi can automatically adjust the growth temperature of the fungi.