The invention discloses a smooth muscle antibody detecting method. The smooth muscle antibody detecting method comprises, firstly, collecting and storing a sample, and preparing the sample and reagents; secondly, after the sample is fed, downwards putting the side of a slide, which is covered by an organism slice, to cover the groove of a sample feeding plate for primary incubation at room temperature; thirdly, in a flowing mode, washing the slide incubated in the second step in a beaker containing phosphate buffer solution, then immersing the washed slide into a small beaker containing phosphate purging liquid, uniformly blending fluorescein and antihuman globulin marking conjugates, adding 20 ul of the mixture into a clean sample feeding plate reaction area, and performing incubation after second antibodies are completely added; fourthly, sucking up water on the back surface and the four sides of the sample-fed slide in the third step for secondary incubation at room temperature; fifthly, in a flowing mode, washing the slide incubated in the fourth step in a beaker containing phosphate buffer solution, then immersing the washed slide into a small beaker containing phosphate purging liquid, and sealing up the slide; sixthly, performing result judgment and interpretation. The smooth muscle antibody detecting method is scientific in process, accurate in standard, good in practical operating effects and high in accuracy.