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发明公开
CN109486818A 狗尾草U6启动子基因及应用 有权 转让

狗尾草U6启动子基因及应用
摘要：
本发明公开了2种狗尾草U6启动子及应用，该2种启动子序列如SEQ ID NO.1、SEQ ID NO.2所示，通过GUS染色瞬时表达验证，能高效的在狗尾草上表达。在转基因技术领域，这些启动子不仅适用于狗尾草，除用于启动功能基因表达外，在构建RNAi表达载体时可用于启动发夹结构的表达，在CRISPR/Cas9系统中可用于启动sgRNA引导序列的表达等。
摘要（英）：

The invention discloses a green bristlegrass herb U6 promoter gene and an application. Sequences of two promoters are as shown in SEQ ID NO.1 and SEQ ID NO.2, the promoters can be efficiently expressed on green bristlegrass herbs through GUS staining instantaneous expression verification and are applicable to the green bristlegrass herbs in the technical field of transgenosis, the promoters are used for promoting expression of functional genes and can further be used for promoting expression of a hairpin structure when a RNAi expression vector is built, and the promoters can be used for promoting expression of an sgRNA guide sequence and the like in a CRISPR/Cas9.

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