The invention relates to the field of machining equipment of insulation bottles, in particular to a cover tightening mechanism for insulation bottle production. The mechanism comprises a power distribution control box; a loading device, a covering device and a tightening device are arranged on the power distribution control box from left to right; the loading device comprises a loading lifting cylinder and a loading lifting base; the loading device is matched with the covering device through a taking device; the covering device is matched with the tightening device through a feeding device; and the taking device, the covering device, the feeding device, the tightening device and the loading lifting cylinder are connected to the power distribution control box. The cover tightening mechanismfor insulation bottle production uses the loading device for loading, realizes cooperation between the loading device and the covering device through the taking device, and meanwhile, can feed sleeved bottle bodies and bottle covers to the tightening device for tightening through the feeding device to realize integral automatic operation, so that the assembly efficiency is greatly improved, and meanwhile, the product quality is improved.