The invention discloses a method for breeding a queen bee, which comprises the following steps: step 1, investigating the swarm situation of a base, and determining the time of queen bee breeding according to the local climate and nectar source conditions; step 2, choosing a strong group during overwintering as a parent group, and taking precious good care until a spring propagation period; step 3, during the spring propagation period, selecting high-quality beeswax as a queen bee platform, sterilizing wax sticks with boiling water, and sterilizing a grafting needle with alcohol for standby application; step 4, putting the queen bee platform into the middle of the parent group, moving the queen bee away or isolating the queen bee from the queen bee platform with a queen bee cage, and sealing the parent group in the queen bee platform to be naturally cleaned for about 1 hour; step 5, grafting larvae with the grafting needle, choosing 1 or 2 old brood combs, and choosing 1-2 days of larvae; and step 6, when drone brood combs are sealed in the parent group, grafting larvae and breeding the queen bee, and conducting mating to obtain a new queen bee. A brand-new generation of apis cerana queen bee with cold resistance, high temperature resistance, strong disease resistance, rapid reproductive capacity, strong acquisition, and strong adaptability is bred, and the quality of the queenbee is improved.