The invention discloses an assembling device of bicycle axle accessories. The assembling device comprises a chain, a first lifting turnplate, a second lifting turnplate, a first runner and a second runner. Side plates are fixed to chain links of the chain at equal intervals. First rollers and second rollers are mounted on the side plates correspondingly. First slide blocks are mounted on a turnplate surface of the first lifting turnplate, and compressed springs are arranged between the first lifting turnplate and the first slide blocks in a matched mode correspondingly. Grooves are machined inthe first slide blocks and used for accommodating lock nuts, jam nuts, shaft sleeves and bead crosspieces correspondingly. A first air cylinder is fixed to a position above the first lifting turnplate. Second slide blocks are mounted on the turnplate surface of the second lifting turnplate, and compressed springs are arranged between the second lifting turnplate and the second slide blocks correspondingly. Grooves are machined in the second slide blocks and used for accommodating lock nuts, jam nuts and bead crosspieces correspondingly. A second air cylinder is fixed to a position above the second lifting turnplate. The device is compact in structure and controlled by a PLC in a unified mode, the axle accessories can be automatically assembled by means of the device, the human capital isreduced, the assembling efficiency is improved, and the profit space is expanded.