The invention discloses a method for tumor marker quantitative determination through a metal-wrappage -antibody composite nano particle based on raman enhancement. The method comprises the following steps of: dispersing a metal nano particle included in an inorganic substance or an organic substance or a polymer or a precious metal into a buffer solution and adding an antibody to realize reaction,enabling the antibody to wrap the metal nano particle, thereby obtaining the metal-wrappage -antibody composite nano particle, and dispersing the metal-wrappage -antibody composite nano particle in the buffer solution after washing; and repeatedly preparing various metal-wrappage -antibody composite nano particle solution containing different antibodies and having different concentrations, and uniformly mixing the solution; mixing purified liquid containing a tumor marker with the nano particle mixed solution, after reaction, obtaining a new metal-wrappage -antibody-antigen composite nano particle formed after specific binding of the antibody and the antigen, then, performing transmission spectrum detection, and obtaining concentration of various tumor markers. The method provided by theinvention has the advantages of being simple to operate and highly sensitive and having wide detection intervals and strong specificity. The method can simultaneously perform quantitative analysis forvarious tumor markers, and has great significance in early diagnosis of tumor.