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发明公开
CN109207420A 人造皮肤的制造方法及人造皮肤 审中-实审

人造皮肤的制造方法及人造皮肤
摘要：
一实现例提供一种人造皮肤的制造方法，该方法包括以下步骤：通过对成纤维细胞及胶原蛋白进行混合而形成仿真皮层；在所述仿真皮层上应用角质形成细胞后进行培养；在第一培养基中培养所述仿真皮层，所述第一培养基为角质形成细胞培养基；及将在所述第一培养基中培养的仿真皮层在混合DMEM培养基及F12培养基而成的第二培养基中进行培养。
摘要（英）：

An embodiment provides a producing method of artificial skin. The method of producing artificial skin includes mixing fibroblasts and collagen to form a dermis simulating layer, applying keratinocyteson the dermis simulating layer and culturing the same, culturing the dermis simulating layer in a first medium that is a keratinocyte medium, and culturing the dermis simulating layer that is cultured in the first medium in a second medium including a DMEM medium and a F12 medium.

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