The invention discloses a manufacturing process of a display screen mask and a display screen. The process comprises the following steps: S1, providing a light board provided with LED lamp beads; S2,arranging a rubber blocking frame, wherein the light board is located in a hollow region of the rubber blocking frame and the peripheral wall of the light board is attached to the inner wall surface of the rubber blocking frame; S3, filling a glue I to the hollow region of the rubber blocking frame, and making the glue I covering the lamp beads; S4, providing a pressure plate, arranging a separation layer on the upper surface of the glue I or on the bottom surface of the pressure plate close to the glue I, and using the weight of the pressure plate to extrude excess glue I from the gap betweenthe rubber blocking frame and the pressure plate; S5, solidifying the glue I; S6, removing the pressure plate and the rubber blocking frame. Additionally assembling the mask and the light board is not needed, the mask will not damage the lamp beads is ensured, and yield of the display screen is improved. The gap between the lamp beads is completely filled by the mask, so that the lamp beads are fully protected.