The invention relates to the field of biological sample analysis and detection equipment, in particular to a chemiluminescence detector. The chemiluminescence detector comprises a sample injection device, a reaction cup supply and recovery device, a reagent disk, an incubation disk component, a detection chamber and a manipulator system. The reaction cup supply and recovery device can automatically open a reaction cup and is matched with a reagent grabbing arm device to add the reaction cup in the incubation disk component. The reagent disk is used for providing a reagent and is matched with the reagent grabbing arm device to add the reagent in the reaction cup on the incubation disk component. The sample injection device is used for conveying a sample test pipe and is matched with a sample arm device to add a sample in the reaction cup on the incubation disk component so as to react with the reagent; in addition, after being subjected to a series of treatments by the incubation disk component, the reaction cup is conveyed to the detection chamber for detection. The chemiluminescence detector provided by the invention has the benefits that the degree of automation in the whole process is high, the intensity of manual operation is reduced, and the risk of operator exposure to medical wastes is reduced.