The invention relates to the technical field of probiotic preparations, in particular to a lactic acid bacteria compound with an intestinal flora adjusting function and a preparation method of the compound. The lactic acid bacteria compound is prepared from 30-50 parts of lactobacillus acidophilus dry powder, 20-35 parts of streptococcus thermophilus dry powder, 15-30 parts of lactobacillus reuteri dry powder, 30-40 parts of bifidobacterium lactis dry powder, 25-35 parts of bifidobacterium longum dry powder, 15-25 parts of lactobacillus rhamnosus dry powder, 6-15 parts of fructo-oligose, 5-10parts of xylooligosaccharide and 8-20 parts of natto powder by mixing. The best scientific ratio is adopted, all components are matched with each other to achieve synergistic effects, intestinal tractnormal flora of a body can be jointly adjusted, micro-ecological balance is kept, the lactic acid bacteria compound is good in stability, long in quality guarantee period and low in sugar content within a long time, and the preparation method is simple and convenient in operation, reliable in running, high in production benefit and applicable to industrial production.