A vibration and noise reduction structure of a highway tunnel includes tunnel segments and ballast beds, A first arc-shaped steel bar and a second arc-shaped steel bar are equally spaced between the ballast bed and the tunnel segment, A first arcuate steel bar is oriented opposite to a second arcuate steel bar, At that bottom of the tunnel segment, a steel pipe pile is penetrate between the adjacent first arc-shaped steel bar and the second arc-shaped steel bar, A dome form is arranged on the inner side of the tunnel segment, A metal mesh is arrange on that outer side wall of the dome form andthe inn wall of the tunnel segment, A lay of sound-absorbing material is sandwiched between that two metal mesh, the invention also discloses a construction method of the vibration and noise reduction structure of the highway tunnel, At that same time, the first arc-shape steel bar and the second arc-shaped steel bar have certain deformation performance and certain vibration damping effect, and the sound-absorbing material lay is arranged on the tunnel wall, so that the sound-absorbing effect is achieved, and the noise brought by echo is reduced.