The invention discloses an extraction method of lutein in pumpkin and application thereof. An ultrasonic assistant solvent extraction method is adopted, cell walls of the pumpkin are instantaneously broken by utilizing the vast pressure generated by the ultrasonic waves, the release and dissolution of content in the cells can be accelerated by virtue of an ultrasonic mechanical effect and heat effect, the extraction time is shortened, the saponification reaction is accelerated, the preparation time of the lutein is shortened, the decomposition of lutein caused by the long-time preparation canbe avoided, and the extraction rate can be improved; ethyl acetate and absolute ethyl alcohol are used as extraction agents to substitute the traditional extraction agent tetrahydrofuran, acetone andpetroleum ether, so that the preparation safety of the lutein can be improved; moreover, the pumpkin planting area is large, the price is low, the pumpkin is rich in lutein, compared with the conventional extraction material marigold flower of the lutein, the pumpkin is wide in source and convenient and easy to obtain and convenient to store, and the production cost is low; and the extraction rateof the pumpkin lutein extracted by utilizing the extraction method of the invention can reach 83.22 percent and is far higher than the plant lutein extraction rate in the prior art, and the development value is high.