An embodiment of the present invention provides a radio frequency communication module, Mobile terminal and preparation method thereof, The radio frequency communication module comprises: a housing, substrate, radio frequency communication chip, first antenna interface and antenna interface connection structure. The radio frequency communication chip and the first antenna interface arranged on thesubstrate, the top of the housing is provided with a through hole. One end of the antenna interface connection structure is electrically connected with the first antenna interface, and the other endof the antenna interface connection structure is used for electrically connecting with the metal case of the mobile terminal through the through hole. In accordance with an embodiment of that presentinvention, the first antenna interface of the radio frequency antenna interface connection structure is arranged on the substrate, the number of antenna shrapnel originally arranged on the system motherboard of the mobile terminal can be reduced, the area of the system motherboard can be effectively reduced, and the internal space of the mobile terminal can be saved.