The invention discloses a temperature sensor calibration method against the reverse Early effect. The temperature sensor calibration method against the reverse Early effect comprises the following steps: applying an input voltage V to an ADC input terminal; calculating [Delta]V(T<1>) and V(T<1>) at a current first temperature according to the read ADC output at the first temperature; calculating [Delta]V(T<2>) and V(T<2>) at a current second temperature according to the read ADC output at the second temperature; and obtaining an actual temperature coefficient in the V by combining the [Delta]V(T<1>), the V(T<1>), the [Delta]V(T<2>) and the V(T<2>), and comparing with an ideal temperature coefficient to obtain a temperature coefficient error in the V. The temperature sensor calibration method against the reverse Early effect avoids the requirement of providing a high-precision temperature environment during the calibration of a high-precisionsensor, and reduces test condition requirements and test costs.