The invention discloses an upper clamp for photoelectric detection automatic coupling equipment. The upper clamp comprises an L-shaped adapter plate, an air cylinder, an optical fiber fixing base, anoptical fiber fixing cover plate, a TOSA device chuck and a TOSA device limiter, etc. The air cylinder is arranged below the transverse plate of the L-shaped adapter plate. The TOSA device limiter isfixedly arranged below the air cylinder. The TOSA device chuck is arranged at one end of the air cylinder. The air cylinder can make the TOSA device chuck open and close to clamp or loosen the opticalfiber adapter. A limiting groove arranged at the tail end of one end of the TOSA device limiter, the clamping place of the TOSA device chuck, a notch arranged on the optical fiber fixing base and a groove mouth arranged on the optical fiber fixing cover plate are collinear in the vertical direction. Compared with the clamps in the prior art, the upper clamp can realize automatic adjustment of theposition and clamping and loosening of the clamp so as to be convenient and quick and high in efficiency.