The invention discloses an integrated biosensor chip. The integrated biosensor chip comprises a substrate comprising through hole arrays; a plurality of electrode needles inserted into the substrate through holes; a plurality of electrode plating layers used for plating of the upper end surfaces of the electrode needles and forming of electrode units; a plurality of electrochemical reaction promoting enzyme coated layers used for coating the upper surfaces of the electrode plating layers, wherein based on the different of substances to be measured, the enzyme coated layers are used for promoting electrochemical reaction related to the substance to be measured and generating electric signals; a culture separating support which is arranged on the substrate, and is used for forming separatedchambers without electrode plating layer coating, wherein a sensor unit composed of two or more electrode units is arranged in each separated chambers; and a bionic tissue unit which is used for providing bionic tissues and culture liquid environment. The integrated biosensor chip is capable of realizing simultaneous real-time measuring of a plurality of biological signals at different parameter conditions, can be used for high flux medicine screening, pathology research, and therapy research, is short in consumed time, rapid in response, and high in flux.