The invention belongs to the technical field of quality detection of meat products and in particular relates to an identification method for cold fresh beef and unfrozen beef based on an ultrasonic imaging technology. The identification method for the cold fresh beef and the unfrozen beef specifically comprises the following steps: preparing beef samples and encoding; carrying out ultrasonic reflection image acquisition on the beef samples by applying an ultrasonic imaging technology; extracting textural feature values of beef ultrasonic reflection images; carrying out dimension reduction treatment on data by utilizing main component analysis; establishing a rapid identification model of the imported cold fresh beef and the unfrozen beef by adopting a stoichiometry method; carrying out ultrasonic reflection image acquisition on unknown beef samples to be detected, and identifying classes of the unknown beef samples to be detected by utilizing the established identification model. According to the identification method provided by the invention, the samples do not need to be pre-treated so that the detection cost is low and the environment is not polluted; the identification methodis simple to operate and accurate in result. When the cold fresh beef and the unfrozen beef are identified, identification of a training set and a testing test reaches 100 percent when the number of the principal components is 5; rapid identification can be realized.