The invention discloses an isolation structure of an automotive filter. The isolation structure comprises a protection box, wherein the automotive filter is fixedly arranged in the protection box; anopen cover is arranged at the upper part of the protection box; bumps are arranged at two sides of the bottom of the open cover, are located in the protection box and are arranged close to an inner side wall of the protection box; sliding chutes corresponding to the positions of the bumps are arranged on the inner side wall of the protection box; a slide bar is connected with the inside of each sliding chute in a sliding manner; a limiting groove corresponding to the end part of the corresponding slide bar is arranged in each bump; and the protection box is provided with chambers below the sliding chutes. The automotive filter is fixedly arranged in the protection box and is in a sealed state to play waterproof and dustproof roles through the arrangement of the open cover; installation andremoval of the open cover are relatively simple; a sealing rubber ring pasted at the bottom of the open cover is easy to wear after being used for a relatively long period of time, so that the sealing rubber ring is convenient to replace through removing the open cover; and the isolation structure of the automotive filter is worthy of vigorous promoting.