知搜-全球专利检索

  1. 登录
  2. 注册
发明授权
CN108828030B 基于AuNPs增强Na2S2O8/O2的ECL效应的蛋白激酶检测方法 失效 - 权利终止

基于AuNPs增强Na2S2O8/O2的ECL效应的蛋白激酶检测方法
摘要：
本发明公开了一种基于AuNPs增强Na2S2O8/O2的ECL效应的蛋白激酶检测方法，属于电致化学发光技术领域。将多肽修饰于玻碳电极表面，然后在蛋白激酶和巯基三磷酸腺苷的作用下，使得多肽发生磷酸化反应，之后加入AuNPs，通过Au‑S键作用将AuNPs连接到多肽的巯基磷酸化位点上，从而拉近了AuNPs与电极表面的距离，AuNPs良好的电子传导性使得Na2S2O8/O2体系的ECL信号大大增强，且ECL增强的强度与蛋白激酶浓度呈正相关，据此可实现蛋白激酶活性的高灵敏和选择性检测，并用于对蛋白激酶抑制剂的筛选。
摘要（英）：

The invention discloses a protein kinase detection method based on AuNPS enhanced Na2SO8/O2 ECL (electrogenerated chemiluminescence) effect, and belongs to the technical field of electroluminescence.The method comprises the following steps: the surface of a glassy carbon electrode is modified with polypeptide, polypeptide is subjected to phosphorylation reaction under the action of protein kinaseand mercaptoadenosine triphosphate, and AuNPs is then added and bound to a mercapto phosphorylation site of polypeptide under the action of Au-S bonds, therefore, the distance between AuNPS and the electrode surface is shortened, the ECL signal of the Na2SO8/O2 system is greatly enhanced by good electron conductivity of AuNPS, the enhancement intensity of ECL is positively correlated with the concentration of protein kinase, high-sensitivity and high-selectivity detection of protein kinase activity can be achieved, and the method is used for screening protein kinase inhibitors.

公开/授权文献
0/0