The invention relates to a preparation method of a high-adhesiveness conductive adhesive, and belongs to the technical field of preparation of electronic materials. According to the method, firstly bamboo fiber is soaked through dopamine, and a polydopamine film with metal ion chelation is formed on the surface of the bamboo fiber under the action of dissolved oxygen in water. According to the method, collagen in scales of silver carps is extracted, the collagen has plenty of neutral amino acid and acidic amino acid, has the effect of anion aggregation, can properly absorb heavy metal, furtherhas a characteristic of antioxidant activity, ensures that heavy metal components with electric conduction effect in conductive adhesive is not easy to oxidize, the method improves the adhesiveness of the conductive adhesive through the collagen, the collagen comprises various collagen chains, the collagen chains are intertwined to form a helical structure, the structure is dense, side groups anda material substrate can perform hydrogen bond adsorption, charge adsorption and other chemical bonding force, the production of cohesive force ensures that the adhesiveness of the conductive adhesive is greatly improved, and the method has a wide application prospect.