The invention relates to biological experimental devices, in particular to a cleaning device for a tissue culture bottle. The device comprises a box body, electric telescopic rods, an erecting cylinder, a transmission device and a control device; the box body comprises a cleaning bin and a containing bin, an ultrasonic generator is arranged in the containing bin, and heating pipes are arranged atthe top of the cleaning bin; the heating pipes are fixedly connected with the inner wall of the cleaning bin, a plurality of culture bottle fixing frames are arranged on the erecting cylinder, and twoelectric telescopic rods are arranged on the two sides of the inner wall of the cleaning bin, one ends of the electric telescopic rods are fixedly connected with the cleaning bin, and the other endsof the electric telescopic rods are rotationally connected with the two ends of the erecting cylinder through connecting rods; and the transmission device is used for driving the erecting cylinder, and the control device is used for controlling the cleaning device to operate. According to the device, the invention provides the cleaning device for the tissue culture bottle, the tissue culture bottle can be effectively cleaned and dried, and a tissue waste can be treated.