The invention provides Pleurotus eryngii with hot-sour taste, and a preparation method thereof, wherein the Pleurotus eryngii with hot-sour taste comprises, by weight, 50-70 parts of Pleurotus eryngii, 20-25 parts of homemade hot and sour sauce, 3-5 parts of sugar, 4-7 parts of flower oil, 1-2 parts of monosodium glutamate, 8-10 parts of edible salt, 3-7 parts of water, and 4-6 parts of starch. The preparation method comprises: (1) material selection, (2) pretreatment in oil, (3) seasoning, (4) sterilization, and (5) packaging. According to the present invention, preservatives and chemical seasonings are not added during the production of the Pleurotus eryngii with hot-sour taste, such that the taste of Pleurotus eryngii is unique, the Pleurotus eryngii integrates convenient carrying and long-time storage while the taste is rich, the shelf life is up to more than 12 months, and the obtained product further has chewing and crisp taste, and is the environmentally friendly, healthy, safeand organic snack food.