The invention relates to the technical field of biomedical instruments, and particularly discloses a double-track cryopreservation pipe assembling device which comprises a cover feeding machine, a cover pressing machine, a cover tightening machine, a pipe discharging machine and a feeding machine. The cover feeding machine comprises a first circular arc conveying track, a second circular arc conveying track, a third circular arc conveying track, a fourth circular arc conveying track and a fifth circular arc conveying track; the cover pressing machine comprises a T-shaped plate, a through hole,an air blowing pipe, a first air cylinder, a metal plate and a photoelectric sensor switch; the cover tightening machine comprises a second air cylinder, a drive mechanism and a third air cylinder; the drive mechanism comprises a first drive motor and a rotating mechanism; the pipe discharging machine comprises a fourth air cylinder, a discharge hopper, a push plate, a horizontal baffle plate andan inclined baffle plate; the feeding machine comprises a rotating plate and a divider; and an installation column, an assembling hole, a pull rod, a push block and a third spring are arranged on therotating plate. The double-track cryopreservation pipe assembling device provided by the invention has the characteristics of high conveying efficiency, low labor cost, no unqualified products, highpressing efficiency and high automation degree.