The invention discloses a method for comprehensively utilizing waste tobacco leaves. The method comprises the steps of after using an organic solvent to extract the waste tobacco leaves, using a stepwise solvent extraction method to obtain condiments, chlorogenic acid, and precipitates of a nicotine extract and rutin; obtaining high-purity nicotine, condiments, solanesol, rutin and chlorogenic acid by using separation and purification means of solvent extraction, column chromatography, crystallization and the like; fermenting tobacco leaf residues of the extracted effective components to prepare a bacterial manure. The method provided by the invention can realize high-value utilization of effective components of multiple waste tobacoo leaves, is simple in process and low in cost, and meetsthe large-scale industrial production requirement; the yield and the purity of the effective components are high, the condiments do not contain the nicotine, and microbial fertilizer is short in production period and high in viable count, so that the method is a tobacco leaf comprehensive utilization technology with high efficiency, good economy and environment friendliness.