The invention relates to a colla carapacis preparation process. The colla carapacis preparation process includes steps: S1, carapax et plastrum testudinis pretreatment; S2, crushing; S3, extracting; S4, gelatin combining; S5, separating and concentrating; S6, skimming; S7, blending, collecting and gelling; S8, cutting and airing. The step of carapax et plastrum testudinis pretreatment includes: applying NaOH solution to carrion parts of carapax et plastrum testudinis, performing ultrasonic treatment for 5min and microwave treatment for 1-2min, repeating for 2-3 times, putting into clear waterafter the last-time microwave treatment is finished, ultrasonically treating for 5min, and cleaning. By adoption of the colla carapacis preparation process, the colla yield of carapax et plastrum testudinis can be effectively increased, and time shortening and product quality improvement are realized. A novel method is provided for colla carapacis preparation.