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发明公开
CN108659068A 一种矢车菊素-3-O-芸香糖苷的分离纯化方法及其应用 无效 - 撤回

一种矢车菊素-3-O-芸香糖苷的分离纯化方法及其应用
摘要：
本发明公开了一种矢车菊素-3-O-芸香糖苷的分离纯化方法及其在制备α-葡萄糖苷酶抑制剂中的应用，该分离纯化方法包括粗提、大孔树脂吸附、高速逆流色谱分离及固相萃取纯化，通过将高速逆流色谱与固相萃取技术相结合，再通过对工艺参数的优化，从桑椹中分离制备得到高纯度的矢车菊素-3-O-芸香糖苷单体；经活性试验发现，该矢车菊素-3-O-芸香糖苷单体能够显著抑制α-葡萄糖苷酶的活性，可用于制备α-葡萄糖苷酶抑制剂。
摘要（英）：

The invention discloses a separating and purifying method for cyanidin-3-O-rutinoside chloride and application thereof in preparation of alpha-glucosidase inhibitors. The separating and purifying method comprises the following steps of coarse extracting, macroporous resin adsorbing, high-speed reverse spectrum separating, and solid-phase extracting and purifying. The separating and purifying method has the advantages that by combining the high-speed reverse spectrum and solid-phase extracting technique, and optimizing the technology parameters, the high-purity cyanidin-3-O-rutinoside chlorideis separated from fruit of mulberry; the activity testing finds that the cyanidin-3-O-rutinoside chloride monomer can obviously inhibit the activity of alpha-glucosidase, and can be used for preparingthe alpha-glucosidase inhibitor.

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