The invention relates to konjac and mango fresh cheese and a preparation method thereof. The method comprises the following operation steps: 1, performing enzymolysis for konjac refined powder, and performing screening to obtain konjac enzymatic powder; 2, peeling clean mangoes, and making mango juice; 3, adding the konjac enzymatic powder and mango juice into fresh cow milk to obtain reinforced konjac mango fresh cow milk; and 4, adding calcium chloride into the reinforced konjac mango fresh cow milk, adding starter into the mixture, performing acid production, milk curdling, squeezing and after-maturing to obtain the konjac and mango fresh cheese. The konjac and mango fresh cheese has rich fragrance, light yellow color and smooth texture, has better functional characteristic and food safety by only utilizing milk, konjac and mangoes, as well as fermentation of glucomannan; and the chewing performance of fresh cheese can be improved by applying the konjac enzymatic powder, and the texture and taste of the fresh cheese can be improved. The konjac and mango fresh cheese is suitable for eating.