The invention discloses a tissue culture rapid propagation method capable of inducing regeneration of germs of mussaenda pubescens. A main technical key point of the method is that the propagation method comprises the following steps: seeds of the mussaenda pubescens are used as an explant, the seeds are induced to be germinated, adventitious buds are induced, and after strong seedlings are rooted, seedling hardening domestication is performed and transplanting potting is performed. When the tissue culture rapid propagation technology of the regeneration of the germs of the mussaenda pubescensprovided by the invention is adopted for mass production, the regeneration cycle is short, the propagation coefficient is high, and the method is suitable for industrialized scale seedling raising.