The invention discloses a method for manufacturing a three-dimensional small-diameter vessel model. The method comprises the following steps of a, preparation of a supporting bracket: utilizing a silica gel material to perform 3D printing of a supporting bracket with an inner and outer concentric circle shape structure; b, formation of a medial membrane: pouring an accellular extracellular matrixcontaining a human aortic smooth muscle cell into a middle layer of the supporting bracket to form the medial membrane; c, formation of an intima: pouring a human umbilical vein endothelial cell suspension into an inner layer of the supporting bracket to form the intima; and d, formation of an adventitia: wholly putting the supporting bracket in a dynamic culture system fully filled with human newbaby dermal fibroblast suspension to form the adventitia. The vessel model acquired with the method is good in biocompatibility, has relatively strong mechanical property and can be used for pathological model study and drug screening relevant to vessels.