The invention relates to a single-bus segmented wiring mode-based bus-partitioning secondary misoperation-prevention method and device. A current running state of a protection device, a switching state of a press plate, real-time measurement value data of a substation and the like are acquired in real time by a monitoring system, judgment is performed by the misoperation-prevention method, feasible assessment is performed on switching operation of flexible-rigid board of secondary equipment during the running state conversion process of an intelligent substation bus, shutting and warning can be performed on a potential risk and operation which does not conform to rule, the safety operation of the press plate of the protection equipment is ensured, a virtual loop link press plate is prevented, and the inconsistent switching behavior of the functional press plate is protected..