The invention discloses a wiring harness winding mechanism. The wiring harness winding mechanism comprises a sleeve, a first plug, a second plug, a first rotating rod, a second rotating rod, a turntable, a gear sleeve, a baffle plate, a rotating motor, a gear, a positioning frame, a fixed frame and the like. The first rotating rod and the second rotating rod are arranged in the sleeve, and wiringharnesses are wound in the sleeve by using rotation of the two rotating rods, so that the winding fixation of the wiring harnesses on a wiring harness connector is realized, and such problems as shortcircuit caused by contact between the wiring harnesses and a control main board are prevented; through arrangement of the rotating motor and the gear, the gear is driven to operate by the work of therotating motor, so that the turntable is rotated, the first rotating rod and the second rotating rod on the turntable are rotated to drive the wiring harnesses to wind, the winding effect is excellent, and both time and labors are saved; and through arrangement of the positioning frame, the first rotating rod and the second rotating rod can be fixed after the wiring harnesses are wound.